| Title |
Electrochemical measurement for analysis of DNA sequence |
| Authors |
조성보(Jo, Seong-Bo) ; 홍진섭(Hong, Jin-Seop) ; 김영미(Kim, Yeong-Mi) ; 박정호(Park, Jeong-Ho) |
| Keywords |
genotyping ; DNA chip ; double stranded DNA ; methylene blue ; cyclic voltammetry ; chronocoulommetry |
| Abstract |
One of the important roles of a DNA chip is the capability of detecting genetic diseases and mutations by analyzing DNA sequence. For a successful electrochemical genotyping, several aspects should be considered including the chemical treatment of electrode surface, DNA immobilization on electrode, hybridization, choice of an intercalator to be selectively bound to double standee DNA, and an equipment for detecting and analyzing the output signal. Au was used as the electrode material, 2-mercaptoethanol was used for linking DNA to Au electrode, and methylene blue was used as an indicator that can be bound to a double stranded DNA selectively. From the analysis of reductive current of this indicator that was bound to a double stranded DNA on an electrode, a normal double stranded DNA was able to be distinguished from a single stranded DNA in just a few seconds. Also, it was found that the peak reduction current of indicator is proportional to the concentration of target DNA to be hybridized with probe DNA. Therefore, it is possible to realize a sim71e and cheats DNA sensor using the electrochemical measurement for genotyping. |