| Title |
A Frequency-dependent Single Cell Impedance Analysis Chip for Applications to Cancer Cell and Normal Cell Discrimination |
| Authors |
장윤희(Chang, YoonHee) ; 김민지(Kim, Min-Ji) ; 조영호(Cho, Young-Ho) |
| DOI |
https://doi.org/10.5370/KIEE.2014.63.12.1671 |
| Keywords |
Cell impedance analysis ; Cell discrimination ; Cancer cells |
| Abstract |
This paper presents a frequency-dependent cell impedance analysis chip for use in cancer and normal cell discrimination. The previous cell impedance analysis chips for flowing cells cannot allow enough time for cell-to-electrode contact to monitor frequency-dependent impedance response. Another type of the previous cell impedance analysis chips for the cells clamped by membranes need complex sample control for making stable cell-to-electrode contact. We present a new impedance analysis chip using the microchamber array, on which a PDMS cover is placed to make stable cell-to-electrode contact for the individual cell trapped in each microchamber; thus achieving frequency-dependent single-cell impedance analysis without complex sample control. Compared to the normal cells, the magnitude of NHBE cells is 60.07~97.41k{Ω} higher than A549 cells in the frequency range of 95.6 kHz~2MHz and the phase of NHBE is 3.96°~20.8° higher than A549 cells in the frequency range of 4.37 kHz~2MHz, respectively. It is demonstrated experimentally that the impedance analysis chip performs frequency-dependent cell impedance analysis by making stable cell-to-electrode contact with simple sample control; thereby applicable to the normal cell and cancer cell discrimination. |